Cucumber Strigolactone Receptor CsDAD2 and GA3 Interact to Regulate Shoot Branching in Arabidopsis thaliana L.

Previous studies identified that strigolactones (SLs) and gibberellins (GAs) interacted when controlling branching in plant shoots, but the underlying mechanism remains unknown. qRT-PCR analysis suggested SL receptor gene CsDAD2 was significantly upregulated leaves, stems, nodes of cucumber after treatment with 50 mg/L GA3. Furthermore, cloned introduced into wild-type Arabidopsis plants via Agrobacterium-mediated transformation. For CsDAD2-OE lines, endogenous content GA3 subsequently higher at seedling stage, number primary cauline branches also increased maturity stage compared WT. Additionally, GA-related genes were up-regulated first inter-nodes third thus indicating GA metabolically active these tissues. The expression branch inhibitor AtBRC1 decreased as well lines. These findings suggest might have important functions interactions between GAs SLs it can promote accumulation suppress BRC1, hence increasing branching.